《吉他英雄5》的曲目包括3 Doors Down的"Kryptonite"，Arctic Monkeys的"Brianstorm"，Blink-182的"The Rock Show"，Dire Straits的"Sultans Of Swing"，Jimmy Eat World的"Bleed American"，Johnny Cash的"Ring Of Fire"，Megadeth的"Sweating Bullets"，Motley Crue的"Looks That Kill"，Muse的"Plug In Baby"，Nirvana的"Smells Like Teen Spirit"，Queen & David Bowie的"Under Pressure"，Stevie Wonder的"Superstition"，The Killers的"All The Pretty Faces"，The Raconteurs的"Steady As She Goes"，TV On The Radio的"Wolf Like Me"，A Perfect Circle的"Judith"，AFI的"Medicate"，Attack! Attack! UK的"You And Me"，Band Of Horses的"Cigarettes, Wedding Bands"，Beastie Boys的"Gratitude"，Beck的"Gamma Ray"，Billy Idol的"Dancing With Myself"，Billy Squier的"Lonely Is The Night"，Blur的"Song 2"，Bob Dylan的"All Along The Watchtower"，Bon Jovi的"You Give Love A Bad Name"，Brand New的"Sowing Season (Yeah)"，The Bronx的"Six Days A Week"，Bush的"Comedown"，Children Of Bodom的"Done With Everything, Die For Nothing"，Coldplay的"In My Place"，以及Darker My Love的"Blue Day"。